You Want to Calculate the Frequency of the Allele Causing

You want to calculate the frequency of the allele causing sickle cell disorder in your patients,and you know the genotypes of every individual.Assuming the population is not in Hardy-Weinberg equilibrium,which method can be used to calculate allele frequency?

A) allele-counting method
B) genotype proportion method
C) square root method
D) binomial expansion
E) chi-square test