When Preparing a Chest Tube Drainage System for Use on a Patient,what

When preparing a chest tube drainage system for use on a patient,what should the respiratory care practitioner do to the water seal chamber of the drainage system?

A) Fill the chamber with water to the 20 cm H2O line
B) Fill the chamber with water to the 2 cm H2O line
C) Attach the tubing that goes to the chest tube to the water seal chamber
D) Adjust the suction pressure at the wall outlet to full vacuum