The length of DNA spooled around a histone complex is determined by digesting DNA bound to histones with a DNA endonuclease which forms breaks at unbound DNA sequences,releasing fragments whose size and number reveal the spooled length and overall DNA fragment length.Digestion of an eukaryal DNA fragment generated four DNA segments.How long was the original DNA?

Question

Quizplus · Accepted Answer

If the DNA fragment was spooled around histone complexes, it will have formed four nucleosome complexes. Each nucleosome contains approximately 200 bp of DNA. Therefore, the original DNA fragment length would be 4 x 200 bp = 800 bp. Choice D is the only option that matches the calculated length.

The Length of DNA Spooled Around a Histone Complex Is