You are using triple sugar iron agar to differentiate intestinal bacteria.You believe you have isolated Salmonella.What appearance would you see in the culture tube?

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Quizplus · Accepted Answer

Salmonella typically produces a red slant and yellow butt with black H₂S production on triple sugar iron agar. This is due to the fact that Salmonella is able to ferment glucose and lactose, but not sucrose, which causes the acid production that turns the slant yellow. The bacteria also produce H₂S, which reacts with iron in the agar to form a black precipitate. The red color of the slant is due to the alkaline reaction in the presence of sodium thiosulfate.

You Are Using Triple Sugar Iron Agar to Differentiate Intestinal