The CRITICAL gene when constructing suicide plasmids is:

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The sacB gene codes for a toxin that is lethal to many bacteria, but can be counteracted by sucrose. Therefore, suicide plasmids that contain sacB can be selectively used to grow only bacteria that have lost the plasmid, by selecting for sucrose-resistant colonies. This technique is often used for genetic engineering and cloning procedures. The other genes listed are not critical for suicide plasmids.

The CRITICAL Gene When Constructing Suicide Plasmids Is