You are searching for a Staphylococcus aureus mutant which needs lysine in the culture medium in order to grow (Lysine auxotroph).Which media will be necessary your search? (Select all that apply)

Question

Quizplus · Accepted Answer

To search for a Lysine auxotroph mutant of Staphylococcus aureus, we need to use a minimal culture medium that lacks lysine (to select for the auxotrophy) and also contains penicillin (to selectively inhibit the growth of any wild-type bacteria that are present in the initial starter culture). So, choices B and C are not suitable. Choice E is incorrect because we need a culture medium to perform the search. Hence, the best options are A and D, which are minimal culture media without lysine but with penicillin added to inhibit any wild-type bacteria.

You Are Searching for a Staphylococcus Aureus Mutant Which Needs