Pyrosequencing relies on which of the following enzymes? (Select all that apply)

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Quizplus · Accepted Answer

Pyrosequencing is a method of DNA sequencing based on the "sequencing by synthesis" principle, where nucleotides are added one at a time and the DNA strand is synthesized complementarily to the template strand. DNA polymerase is essential for this synthesis process. Luciferase is used in the detection system of pyrosequencing; it produces light in the presence of ATP, which is generated during the incorporation of a nucleotide. ATP sulfurylase is used to recycle the pyrophosphate (PPi) released during nucleotide incorporation into ATP, which is necessary for the luciferase reaction. Restriction endonuclease and DNA ligase are not involved in the pyrosequencing process.

Pyrosequencing Relies on Which of the Following Enzymes? (Select All