What essential principle of PCR appears to have been overlooked in the figure depicting Part B (PCR-based site directed mutagenesis) ?

Question

What essential principle of PCR appears to have been overlooked in the figure depicting Part B (PCR-based site directed mutagenesis)?

Quizplus · Accepted Answer

The figure depicts Part B of PCR-based site directed mutagenesis, which includes amplification of the mutated gene using PCR. However, the figure does not depict the step of generating single-stranded DNA templates by denaturation of the double-stranded template, which is an essential principle of PCR. Without denaturation, the PCR cannot proceed as the polymerase cannot bind to the template efficiently. Thus, the best choice is A. Choices B, C, and D are not relevant to the figure shown.

What Essential Principle of PCR Appears to Have Been Overlooked