The differential reagent (that which will distinguish a positive from a negative result)in an ELISA test is:

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The differential reagent in an ELISA test is the conjugated antibody with catalytic activity, which produces a detectable signal upon binding to the target antigen. The substrate-containing buffer is used to produce a colorimetric or luminescent signal, but it does not differentiate between positive and negative samples. The primary antibody and antibody provided by a patient are used to capture the antigen and do not produce a detectable signal on their own. The wash buffer is used to remove unbound materials and does not have a direct role in discrimination between positive and negative results.

The Differential Reagent (That Which Will Distinguish a Positive from a Negative