Assaying bacterial isolates for resistance genes by molecular methods:
A)can detect unknown resistance genes not yet defined.
B)cannot detect phenotypic resistance due to a combination of mechanisms.
C)means the resistance gene is expressed if found to be present.
D)is currently part of the routine clinical microbiology workflow for all isolates.

Question

Quizplus · Accepted Answer

Molecular methods for resistance genes can only detect those resistance genes that have been characterized and identified,it does not detect novel resistance genes.Also,just because a resistance gene is present in the bacteria it does not mean that it is expressed.Moreover,if resistance is manifested due to a number of different mechanisms (e.g. ,decreased uptake and altered antimicrobial target)and not a single mechanism associated with a single gene,that resistance would be missed.

Assaying Bacterial Isolates for Resistance Genes by Molecular Methods