The medium or media of choice used to recover Legionella is:

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Specimens for culture should be inoculated to two agar plates for the recovery of Legionella,at least one of which is BCYE without inhibitory agents.This medium contains charcoal to detoxify the medium,remove carbon dioxide (CO₂),and modify the surface tension to allow the organisms to proliferate more easily.BCYE is also prepared with N-(2-acetamido)-2-aminoethanesulfonic acid (ACES)buffer and the growth supplements cysteine (required by Legionella),yeast extract,a-ketoglutarate,and iron.A second medium,BCYE base with polymyxin B,anisomycin (to inhibit fungi),and cefamandole,is recommended for specimens such as sputum that are likely to be contaminated with other flora.

The Medium or Media of Choice Used to Recover Legionella