A Laboratory Assistant That Works with Mice Recently Noticed That

A laboratory assistant that works with mice recently noticed that their lymph nodes were swollen and that they had a lesion on their hand that was not there a few weeks ago.The lymph node tissue is sent to the laboratory for culture.The laboratory technologist noticed on the stained sample that there was an organism present that resembled a spiral,gram negative with flagella.The cultures grew no organisms.What is the most likely cause for the discrepancy?

A) The laboratory technologist misidentified the stain artifact as an organism.
B) The laboratory technologist did not culture the sample to media.
C) The organism is not able to grow on media and requires another method for identification.
D) The organism was not allowed to incubate long enough.