Optimal conditions for most mycobacterial cultures are:
A)25°C to 30°C in the dark in an atmosphere of 2% carbon dioxide (CO₂)and high humidity.
B)35°C in the dark in an atmosphere of 5% to 10% CO₂ and high humidity.
C)35°C in the dark in a microaerophilic atmosphere of 10% CO₂ and high humidity.
D)32°C in the dark in an atmosphere of decreased CO₂ and increased humidity.

Question

Quizplus · Accepted Answer

For optimal cultivation of Mycobacterium spp.from most specimens,cultures are incubated at 35°C in the dark in an atmosphere of 5% to 10% CO₂ and high humidity.If specimens are obtained from skin or superficial lesions suspected to contain Mycobacterium marinum or Mycobacterium ulcerans,then an additional set of solid media should be inoculated and incubated at 25°C to 30°C.In addition,a chocolate agar plate or the placement of an X-factor (hemin)disk on conventional media and incubation at 25°C to 33°C are needed for the recovery of Mycobacterium haemophilum.

Optimal Conditions for Most Mycobacterial Cultures Are