The optimal temperature and length of time to incubate a sample suspected of containing a dermatophyte is _____°C for _____ days.
A)25;10
B)25;14
C)30;7
D)30;21

Question

Quizplus · Accepted Answer

Specimens of hair,skin scrapings or biopsies,and nail clippings are usually submitted for dermatophyte culture and are contaminated with bacteria and/or rapidly growing fungi.Samples collected from lesions may be obtained by scraping the skin or nails with a scalpel blade or microscope slide;infected hairs are removed by plucking them with forceps.These specimens should be placed in a sterile container but should not be refrigerated.Mycosel agar,which contains chloramphenicol and cycloheximide,is satisfactory for the recovery of dermatophytes.Cultures should be incubated for a minimum of 21 days at 30°C before being reported as negative.

The Optimal Temperature and Length of Time to Incubate a Sample