Saponin,polypropylene glycol,SPS,and ethylenediaminetetraacetic acid (EDTA)are all components in which blood culture system?

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The Isolator (Wampole Laboratories,Cranbury,NJ)is a commercially available lysis centrifugation system.The Isolator consists of a stoppered tube containing saponin to lyse blood cells,polypropylene glycol to decrease foaming,SPS as an anticoagulant,EDTA to chelate calcium ions and inhibit the complement cascade and coagulation,and a small amount of an inert fluorochemical (Fluorinert,3M Company,St.Paul,MN)to cushion and concentrate the microorganisms during 30-minute centrifugation at 3000 11ea5c86_236c_629c_8438_f591fd7189ae_TB6072_11 .(Figure 67-6).

Saponin,polypropylene Glycol,SPS,and Ethylenediaminetetraacetic Acid (EDTA)are All Components in Which Blood