The proper technique for processing clotted body fluids sent to the laboratory for fungal culture is:

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Any body fluid received in the laboratory that is already clotted must be homogenized to release trapped bacteria and minced or cut to release fungal cells.Either processing such specimens in a motorized tissue homogenizer or grinding them manually in a mortar and pestle or glass tissue grinder allows recovery of bacteria.Hand grinding is often preferred,because motorized grinding can generate considerable heat and thereby kill microorganisms in the specimen.Grinding may lyse fungal elements;therefore it is not recommended with specimens processed for fungi.Small amounts of whole material from a clot should be aseptically cut with a scalpel and placed directly onto media for the isolation of fungi.

The Proper Technique for Processing Clotted Body Fluids Sent to the Laboratory