The first DNA cloning experiment required ALL of the following EXCEPT:

Question

Quizplus · Accepted Answer

DNA polymerase is not required for the initial steps of DNA cloning, which involve cutting DNA with restriction enzymes, inserting the DNA fragment into a plasmid vector, and introducing this vector into competent cells. DNA ligase is used to join the DNA fragments, and restriction enzymes cut the DNA. DNA polymerase is primarily used in DNA replication and PCR, not in the basic process of cloning.

The First DNA Cloning Experiment Required ALL of the Following