The Sanger Method of DNA Sequencing Has Been Automated and Uses

The Sanger method of DNA sequencing has been automated and uses a(n) :

A) thermal-stable DNA polymerase to allow for multiple rounds of DNA synthesis.
B) trideoxynucleotides in the synthesis reactions.
C) radiolabeled nucleotides for fragment detection.
D) agarose gel electrophoresis for fragment separation.
E) chemical digestion method for fragment generation.