For optimal expression of a eukaryal gene in bacteria,the expression vector must contain all of the following except one.Which would it NOT include?

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Quizplus · Accepted Answer

Eukaryal genes contain introns that need to be spliced out before translation. Bacteria lack the machinery to efficiently remove introns, therefore, including a protein to remove them would not be sufficient for optimal expression in bacteria. Instead, the gene should be modified to remove the introns before cloning into the expression vector. The necessary components for optimal expression in bacteria include a bacterial promoter, a bacterial termination sequence, a bacterial ribosomal binding site, and optionally, a regulatable promoter to control expression levels.

For Optimal Expression of a Eukaryal Gene in Bacteria,the Expression