A his-tag or strep-tag is part of the cloning area in expression vectors.How do these sequences function?
A) To increase gene expression from the cloned DNA.
B) In regulation of expression of the cloned DNA.
C) As unique restriction enzyme sites to aid in cloning DNA.
D) Affinity sequences to aid in purification of the recombinant protein.
E) As signal sequences for the secretion of the recombinant protein.
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