During glycolysis,the transfer of phosphate from phosphoenolpyruvate to ADP is an example of substrate-level phosphorylation.

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Substrate-level phosphorylation occurs when a phosphate group is transferred from a phosphorylated substrate directly to ADP or GDP, forming ATP or GTP. In the case of glycolysis, phosphoenolpyruvate donates a phosphate group to ADP to form ATP, which is an example of substrate-level phosphorylation.

During Glycolysis,the Transfer of Phosphate from Phosphoenolpyruvate to ADP Is