Performance of the "factor requirement test" for Haemophilus involves the:

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Traditional identification criteria include the requirement for X and/or V factors for growth.To establish X and V factor requirements, disks impregnated with each factor are placed on unsupplemented media, usually Mueller-Hinton agar or trypticase soy agar, that has been inoculated with a light suspension of the organism.After overnight incubation at 35° C in ambient air, the plate is then examined for growth around each disk.Many X factor-requiring organisms are able to carry over enough factor from the primary medium to give false-negative results (i.e., growth occurs at such a distance from the X disk as to falsely indicate that the organism does not require the X factor).

Performance of the "Factor Requirement Test" for Haemophilus Involves The