How can a researcher determine if the mutations to the RNA polymerase complex or the mutations in the rho gene were caused by the growth in increased temperatures or if they were merely selected for by growing the ancestral E.coli in the warmer temperatures?

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The Answer of How can a researcher determine if the mutations to the RNA polymerase complex or the mutations in the rho gene were caused by the growth in increased temperatures or if they were merely selected for by growing the ancestral E.coli in the warmer temperatures?

How Can a Researcher Determine If the Mutations to the RNA