Tracheostomized patients in the medical intensive care unit have been developing infections from an unidentified source.What method would the respiratory therapist use to culture the aerosol tubing?

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The rinse sampling technique involves sloshing sterile broth aseptically in respiratory therapy equipment tubing,recovering it,and allowing the broth to incubate.Colonies are then counted and colonizing organisms identified.This method is quantitative and very sensitive.Procedures must be carefully followed so as not to introduce sampling errors.This method is used primarily to culture processed equipment for assessing the effectiveness of the processing method.

Tracheostomized Patients in the Medical Intensive Care Unit Have Been