When Preparing a Chest Tube Drainage System for Use on a Patient,what

When preparing a chest tube drainage system for use on a patient,what should the respiratory care practitioner do to the water seal chamber of the drainage system?

A) Fill the chamber with water to the 20 cm H₂O line
B) Fill the chamber with water to the 2 cm H₂O line
C) Attach the tubing that goes to the chest tube to the water seal chamber
D) Adjust the suction pressure at the wall outlet to full vacuum