How does one begin to identify a predicted protein?

Question

Quizplus · Accepted Answer

To identify a predicted protein, one can compare its predicted amino acid or DNA sequence to that of known proteins using bioinformatic tools such as BLAST or Pfam. This allows for the identification of homologous proteins and can provide insight into the potential function of the predicted protein. Experimental determination of the amino acid sequence would require purification and sequencing of the protein, which is not always feasible. Analyzing the RNA sequence would only provide information on the coding region, not the actual amino acid sequence of the protein. Determining the sequence of its paralog would be useful in identifying similar proteins, but not necessarily in identifying the predicted protein itself.

How Does One Begin to Identify a Predicted Protein