If your goal is to isolate Gram-negative bacteria from Gram-positive bacteria and you are not interested in biochemical differences between the Gram-negative bacteria,it would be best to use a growth media that is

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A selective media will inhibit the growth of Gram-positive bacteria, allowing for the isolation of only Gram-negative bacteria without the need for biochemical differentiation. A differential media would allow for differentiation between types of Gram-negative bacteria, but is not necessary for the isolation of only Gram-negative bacteria. A selective and differential media might be useful if biochemical differentiation was desired, but is not necessary for simply isolating Gram-negative bacteria. A nonselective media would not inhibit the growth of Gram-positive bacteria and therefore would not achieve the goal of isolating Gram-negative bacteria.

If Your Goal Is to Isolate Gram-Negative Bacteria from Gram-Positive