The Image Shown Is a Gel from a Multiplex Polymerase

The image shown is a gel from a multiplex polymerase chain reaction (PCR) used to identify toxin genes in Clostridium botulinum.Which of the following statements most accurately describes how this image was formed?

A) An amplified sample of DNA was combined with four sets of PCR primers.
B) Four patient samples were combined with one set of PCR primers.
C) A thermocycler was used to run four separate PCR amplification cycles.
D) A patient sample was combined with four sets of PCR primers.