Which type of microscope is used to observe acid-fast bacteria when they are stained with auramine and rhodamine?

Question

Quizplus · Accepted Answer

Auramine and rhodamine are fluorochromes (dyes that absorb ultraviolet light and emit light of higher wavelengths),and the fluorescent microscope is used to detect the emitted light.This method is more rapid than the Kinyoun or Ziehl-Neelsen because the slides can be screened using lower magnifications (10× and 40×).The acid-fast bacteria will fluoresce and stand out as a bright yellow/orange color.In the other two methods a brightfield microscope is used and the oil immersion objective (100×)is needed to observe the bacteria.

Which Type of Microscope Is Used to Observe Acid-Fast Bacteria