The technologist uses TSI and LIA agars to rule out stool pathogens,Salmonella and Shigella spp.The reactions observed are TSI: K/A H2S+ and LIA: K/K.What should the technologist do next?

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The reactions are suspicious of Salmonella spp.,and further biochemical and serological testing should be done to confirm the TSI and LIA screening.The TSI: K/A indicates a glucose fermenter,lactose and sucrose nonfermenter,and an H2S producer.The LIA: K/K indicates a negative lysine deaminase and a positive lysine decarboxylase.Both are suspicious of Salmonella.

The Technologist Uses TSI and LIA Agars to Rule Out