Positional cloning
A) uses random cloning to isolate genes.
B) starts by sequencing the entire chromosome containing the gene of interest.
C) makes use of particular markers on the DNA.
D) makes use of the location of Alu sequences.
E) All of these are correct answers

Question

Quizplus · Accepted Answer

Positional cloning makes use of particular markers on the DNA, such as restriction fragment length polymorphisms (RFLPs) or single nucleotide polymorphisms (SNPs), to isolate the gene of interest. The markers are used to identify genomic regions that are likely to contain the gene, allowing for targeted cloning and sequencing. Random cloning (A) is not typically used in positional cloning approaches. While sequencing (B) can be useful for identifying candidate regions, it may not be practical for sequencing entire chromosomes. Alu sequences (D) are a type of repetitive DNA element that can be used as markers, but may not be specific enough for targeted cloning.

Positional Cloning