Which of the following techniques was used to isolate the flavivirus Hepatitis C virus?

Question

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Molecular cloning of the viral cDNA was used to isolate the Hepatitis C virus. This technique involves reverse transcription of viral RNA into cDNA, followed by cloning the cDNA into a vector for further analysis and characterization. Purification of virus particles, antibody staining with patient serum, plaque assay, and Southern blotting are other techniques that can be used for the detection and quantification of viruses, but they are not necessarily involved in the isolation of the virus itself.

Which of the Following Techniques Was Used to Isolate the Flavivirus