All of the Following Factors Can Affect the Efficiency of Transcription

All of the following factors can affect the efficiency of transcription of the mRNAs in the Ebola virus EXCEPT….

A) Strength of the polymerase binding site.
B) Location of the gene in the genome.
C) RNA secondary structure at 3' end of gene.
D) RNA secondary structure in the intergenic region.
E) Presence of overlapping genes.