If the step involving iodine were left out of a Gram stain,which of the following would best describe the results
A)Gram negative cells would look Gram positive.
B)Gram positive cells would look Gram negative.
C)All rods would be pink,all cocci purple.
D)All cells would be purple.

Question

Quizplus · Accepted Answer

The iodine step is crucial in the Gram staining process as it helps to lock in the crystal violet stain in the thick peptidoglycan layer of Gram-positive cells. Without this step, the crystal violet can easily be washed away during the decolorizing step, leading to false-negative results for Gram-positive cells. Therefore, without the iodine step, Gram-positive cells would look Gram negative.

If the Step Involving Iodine Were Left Out of a Gram