Select the limitations of the DNA polymerases used for DNA replication.
A) DNA polymerase can attach new nucleotides only in the 5′ to 3′ direction.
B) DNA polymerases must begin synthesis using a primer or existing DNA strand.
C) DNA polymerases must have a template strand to copy from.
D) DNA polymerases can attach new nucleotides in the 3′ to 5′ direction only on the lagging strand.

Question

Quizplus · Accepted Answer

DNA polymerases can only add nucleotides in the 5′ to 3′ direction, require a primer or existing strand to start synthesis, and need a template strand to copy from. They cannot add nucleotides in the 3′ to 5′ direction.

Select the Limitations of the DNA Polymerases Used for DNA