What is the purpose of RNaseH in the making of cDNA?
A) It copies the RNA into a complementary DNA strand
B) It reforms the sugar-phosphate backbone
C) It generates short RNAs that are used as primers
D) It proofreads the cDNA for errors

Question

Quizplus · Accepted Answer

RNaseH degrades the RNA strand of an RNA-DNA hybrid, which allows for the generation of short RNA primers that are used by DNA polymerase to synthesize the complementary DNA strand.

What Is the Purpose of RNaseH in the Making of CDNA