What occurs during the annealing stage of a PCR reaction?
A) The DNA strands separate
B) The DNA polymerase copies the template DNA.
C) The primers bind to the template DNA.
D) The reaction stops.

Question

Quizplus · Accepted Answer

During the annealing stage of a PCR reaction, the temperature is lowered, allowing the primers to bind to the complementary regions of the template DNA. This is a crucial step in the PCR process as it enables the DNA polymerase to start copying the template DNA. The annealing temperature is specific for each set of primers and is optimized to ensure efficient and specific binding.

What Occurs During the Annealing Stage of a PCR Reaction