Which technique is best suited to identify DNA-protein interactions?
A) Western blotting
B) PCR
C) gel mobility shift assay
D) restriction enzyme analysis

Question

Quizplus · Accepted Answer

Gel mobility shift assay, also known as electrophoretic mobility shift assay (EMSA), is the most appropriate technique for identifying DNA-protein interactions. In this technique, a labeled DNA molecule is allowed to bind with the protein of interest. The DNA-protein complex is then subjected to gel electrophoresis, and the mobility of the complex is compared to that of the unbound DNA. If a DNA-protein complex is formed, it moves slower in the gel than the unbound DNA because of the increased size and altered shape. This technique provides a direct visualization of the protein-DNA interaction and is widely used for studying transcription factor binding to regulatory elements. Western blotting is used to detect target proteins in a sample, PCR is used to amplify target DNA sequences, and restriction enzyme analysis is used to cut DNA at specific locations. None of these techniques are designed for identifying DNA-protein interactions.

Which Technique Is Best Suited to Identify DNA-Protein Interactions