Robert Koch Is Credited with Proving the Germ Theory of Disease;

Robert Koch is credited with proving the germ theory of disease; that infectious diseases are caused by specific microorganisms.  In developing a series of proofs (Koch's Postulates) ,he was able to determine the causative organism of anthrax was the bacteria Bacillus anthracis.  Which of the following statements does not contribute to Koch's Postulates and the germ theory of disease?

A) The causative organism must be evidenced in each subject presenting with the disease manifestations.
B) Inoculating a healthy subject with the isolated organism must result in the reported disease manifestations.
C) The organism must be isolated,purified and characterized from an infected individual.
D) Each organism from a polymicrobial infection must be isolated and inoculated into a single host.
E) The same species of organism must be isolated from the second inoculated individual.