What would be the most reasonable technique to use to determine if a drug is capable of inhibiting a transcription factor?
A) ChIP assay used with a DNA microarray
B) Mass spectometry of proteins in the cell
C) 2-D gel electrophoresis
D) Antibody microarray

Question

Quizplus · Accepted Answer

ChIP assay (chromatin immunoprecipitation) is a technique that can be used to determine if a drug is capable of inhibiting a transcription factor. This technique can be combined with a DNA microarray to determine which genes are regulated by the transcription factor in question. ChIP assay involves using an antibody to isolate the transcription factor and associated DNA sequences from a cell extract, followed by PCR or microarray analysis to identify the bound sequences. This technique is specific and sensitive, and can provide valuable insight into the mechanisms of transcription factor regulation. Mass spectrometry, 2-D gel electrophoresis, and antibody microarrays are useful techniques for protein analysis, but are not as relevant for determining transcription factor inhibition.

What Would Be the Most Reasonable Technique to Use to Determine