In shotgun sequencing, how is a complete sequence of a genome reconstructed?
A) The entire genome is directly sequenced chromosome by chromosome.
B) The genome is fragmented and sequences of each fragment are arranged so that overlapping regions are identified.
C) The sequences of a large number of individuals is obtained and "averaged" to obtain the complete sequence.
D) The position of each marker and gene are first mapped relative to regions for which the genomic sequence is not known.

Question

Quizplus · Accepted Answer

In shotgun sequencing, the genome is fragmented into small overlapping pieces, and each piece is sequenced individually. The sequences of these pieces are then arranged by identifying overlapping regions, resulting in a reconstruction of the complete genome sequence.

In Shotgun Sequencing, How Is a Complete Sequence of a Genome