In positional cloning, which method is used to identify clones that are progressively closer to a gene of interest?
A) Chromosome painting
B) Cytogenetic mapping
C) Microsatellite analysis
D) Chromosome walking

Question

Quizplus · Accepted Answer

Chromosome walking is the method that is used to identify clones that are progressively closer to a gene of interest. This involves starting with a clone that is known to be close to the gene of interest and then screening a genomic library to find overlapping clones that contain more of the gene's sequence. This process is repeated until the gene itself is identified. Chromosome painting involves labeling specific DNA sequences with fluorescent tags to visualize their location on a chromosome, and cytogenetic mapping involves analyzing the distribution of genetic markers across a chromosome to determine the location of a gene. Microsatellite analysis is a method for determining genetic variation that may be linked to a disease or trait, but it is not used to identify the physical location of a gene.

In Positional Cloning, Which Method Is Used to Identify Clones