You perform a cell free translation experiment like Nirenberg and Matthaei. You start with 60% C and 40% A. What relative amount of radiolabeled proline do you expect in the translated polypeptides?

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Quizplus · Accepted Answer

The codon for proline is CCN (where N can be any nucleotide). The probability of forming CCN with 60% C and 40% A is 0.6 * 0.6 * (0.6 + 0.4) = 0.36 or 36%.

You Perform a Cell Free Translation Experiment Like Nirenberg and Matthaei