In which type of microscopy do dust particles interfere the most?
A) bright-field microscopy
B) dark-field microscopy
C) phase-contrast microscopy
D) interference microscopy
E) fluorescence microscopy

Question

Quizplus · Accepted Answer

Dark-field microscopy relies on the scattering of light by a specimen, and dust particles can scatter the light and interfere with the imaging. In contrast, bright-field and phase-contrast microscopy rely on the absorption and phase shift of light, respectively, so dust particles are less likely to interfere. Interference microscopy is designed to study transparent samples, and fluorescence microscopy uses specific wavelengths of light to excite fluorescent molecules, so both are less likely to be affected by dust particles.

In Which Type of Microscopy Do Dust Particles Interfere the Most