Agarose gel electrophoresis separates pieces of linear DNA based on:

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Agarose gel electrophoresis separates pieces of linear DNA based on their size. Smaller fragments will move faster through the gel matrix and be located closer to the bottom of the gel, while larger fragments will move slower and be located closer to the top of the gel. Charge, sequence, supercoiling, and methylation do not directly impact fragment separation in agarose gel electrophoresis.

Agarose Gel Electrophoresis Separates Pieces of Linear DNA Based On