In the early days of cloning, DNA was __________ into E. coli and then could be transferred to another organism using a __________.

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In the early days of cloning, DNA was transformed into E. coli and then could be transferred to another organism using a shuttle vector. Conjugation is the transfer of genetic material between bacterial cells, but it is not used for cloning. Contigs are contiguous sequences assembled from overlapping DNA fragments, and they are not used for transferring DNA between organisms in cloning. The polymerase chain reaction (PCR) is a technique used to amplify DNA, but it is not used for transferring DNA between organisms in cloning.

In the Early Days of Cloning, DNA Was __________ into E