The Sanger method of DNA sequencing involves __________ that bring about chain termination.

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The Sanger method of DNA sequencing involves the use of 2',3' dideoxyribonucleotides (ddNTPs) which lack the 3' hydroxyl group necessary for the formation of a phosphodiester bond with the next incoming nucleotide during DNA synthesis. As a result, when a ddNTP is incorporated into the growing DNA chain instead of a regular dNTP, it terminates the elongation of the chain. This generates a series of fragments of different lengths that can be separated by gel electrophoresis, revealing the sequence of the original DNA template.

The Sanger Method of DNA Sequencing Involves __________ That Bring