A protein of interest can be specifically detected on western blots; this technique requires:

Question

Quizplus · Accepted Answer

All of the above options are required for Western blotting. Primary antibodies produced against the protein under study will specifically bind to the protein of interest. An enzyme-tagged secondary antibody, directed against the primary antibody, will bind specifically to the primary antibody, allowing for detection. The enzyme in the tagged secondary antibody will produce an easily detectable product, such as a chemiluminescent signal, when reacted with the appropriate substrate.

A Protein of Interest Can Be Specifically Detected on Western