In the yeast two-hybrid analysis technique, in vivo protein-protein interactions are explored. The "bait" and "prey" proteins are translationally fused to the cleaved activation and DNA-binding domains of the GAL4 transcription factor. What is the target gene of GAL4?

Question

Quizplus · Accepted Answer

The target gene of GAL4 is GAL1, which encodes a galactokinase involved in the galactose utilization pathway in yeast. The GAL4 transcription factor binds upstream of the GAL1 promoter region to activate its transcription. Therefore, the yeast two-hybrid assay utilizes the activation of the GAL1 gene as a readout for the interactions between the bait and prey proteins. The other options, lacZ and gadA/B, are not regulated by GAL4 in yeast.

In the Yeast Two-Hybrid Analysis Technique, in Vivo Protein-Protein Interactions