Once a gene encoding a putative transcriptional regulatory protein is identified by sequence annotation, what needs to be done to demonstrate this activity?

Name: Once a gene encoding a putative transcriptional regulatory protein is identified by sequence annotation, what needs to be done to demonstrate this activity?
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The Answer of Once a gene encoding a putative transcriptional regulatory protein is identified by sequence annotation, what needs to be done to demonstrate this activity?

Once a Gene Encoding a Putative Transcriptional Regulatory Protein Is